Assessment of the Purity of Emodin by Quantitative Nuclear Magnetic Resonance Spectroscopy and Mass Balance
10.20307/nps.2019.25.3.222
- Author:
Sojung PARK
1
;
Yu Jin CHOI
;
Giang Hoang DO
;
Eun Kyoung SEO
;
Seunghun HYUN
;
Dongho LEE
Author Information
1. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea. dongholee@korea.ac.kr
- Publication Type:Original Article
- Keywords:
qNMR;
Emodin;
HPLC-UV;
Mass balance
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Emodin;
Fabaceae;
Magnetic Resonance Spectroscopy;
Methods;
Polygonaceae;
Relaxation;
Rhamnaceae;
Salts;
Spectrum Analysis;
Trace Elements
- From:Natural Product Sciences
2019;25(3):222-227
- CountryRepublic of Korea
- Language:English
-
Abstract:
Quantitative nuclear magnetic resonance (qNMR) is a well-established method adopted by international pharmacopoeia for quantitative and purity analyses. Emodin is a type of anthraquinone, well known as the main active component of Fabaceae, Polygonaceae and Rhamnaceae. Purity analysis of emodin is usually performed by using the high-performance liquid chromatography (HPLC)-UV method. However, it cannot detect impurities such as salts, volatile matter, and trace elements. Using the qNMR method, it is possible to determine the compound content as well as the nature of the impurities. Several experimental parameters were optimized for the quantification, such as relaxation delay, spectral width, number of scans, temperature, pulse width, and acquisition time. The method was validated, and the results of the qNMR method were compared with those obtained by the HPLC and mass balance analysis methods. The qNMR method is specific, rapid, simple, and therefore, a valuable and reliable method for the purity analysis of emodin.
