Quantification of the Bioactive Components of the Rhizomes of Curcuma wenyujin and Assessment of Its Anti-inflammatory Effect in Benign Prostatic Hyperplasia-1 Cells
10.20307/nps.2019.25.3.238
- Author:
Chang Seob SEO
1
;
Eunsook PARK
;
Mee Young LEE
;
Hyeun Kyoo SHIN
Author Information
1. Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. hkshin@kiom.re.kr
- Publication Type:Original Article
- Keywords:
Curcuma wenyujin;
Anti-inflammatory effect;
BPH-1 cell;
HPLC-PDA
- MeSH:
Calibration;
Cell Line;
Chromatography, Liquid;
Curcuma;
Enzyme-Linked Immunosorbent Assay;
Humans;
Limit of Detection;
Methods;
Prostatic Hyperplasia;
Rhizome
- From:Natural Product Sciences
2019;25(3):238-243
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, the marker compounds of Curcumae Rhizoma (CR) were simultaneously quantified by high-performance liquid chromatography equipped with a photodiode array detector and the anti-inflammatory effects of CR extract and marker compounds in human benign prostatic hyperplasia epithelial-1 (BPH-1) cell lines were investigated. The marker components (4S,5S)-(+)-germacrone-4,5-epoxide, furanodienone, and germacrone, were separated on Gemini C₁₈ columns (250 mm × 4.6 mm, 5 µm) at 40 ℃ by using a gradient of two mobile phases eluting at 1.0 mL/min. Prostaglandin E₂ (PGE₂) levels in Human BPH-1 cells were determined with an ELISA kit. The coefficients of determination in a calibration curve of each analyte were all 0.9997. The limits of detection and quantification of the three compounds were 0.10 – 0.32 µg/mL and 0.30 – 0.98 µg/mL, respectively. The content of three compounds, (4S,5S)-(+)-germacrone-4,5-epoxide, furanodienone, and germacrone, in the CR sample were found to be 5.79 – 5.92 mg/g, 4.72 – 4.86 mg/g, and 1.06 – 1.09 mg/g, respectively. Regarding pharmacological activity against benign prostatic hyperplasia, CR and its components significantly suppressed PGE₂ levels of BPH-1 cells. The established analysis method will help to improve quality assessment of CR samples and related products. In addition, CR and its components exhibit anti-inflammatory activity in BPH-1 cells, suggesting the inhibitory efficacy of these compounds against the pathogenesis of BPH.
