Anti-oxidative Effect of Piperine from Piper nigrum L. in Caenorhabditis elegans
10.20307/nps.2019.25.3.255
- Author:
Hyun Mee PARK
1
;
Jun Hyeong KIM
;
Dae Keun KIM
Author Information
1. College of Pharmacy, Woosuk University, Jeonju 55338, Republic of Korea. dkkim@woosuk.ac.kr
- Publication Type:Original Article
- Keywords:
Piperine;
Piper nigrum L.;
Caenorhabditis elegans;
Antioxidant activity
- MeSH:
1-Butanol;
Abdominal Pain;
Anorexia;
Caenorhabditis elegans;
Caenorhabditis;
Catalase;
China;
Diarrhea;
Fruit;
Japan;
Korea;
Medicine, Traditional;
Methanol;
Methylene Chloride;
Oxidative Stress;
Piper nigrum;
Piper;
Seasons;
Superoxide Dismutase;
Vomiting
- From:Natural Product Sciences
2019;25(3):255-260
- CountryRepublic of Korea
- Language:English
-
Abstract:
Piper nigrum L. (Piperaceae), which is a well-known food seasoning, has been used as a traditional medicine for the treatment of vomiting, abdominal pain, diarrhea and anorexia in Korea, China and Japan. Methanol extract from the fruit of P. nigrum was successively partitioned as n-hexane, methylene chloride, ethyl acetate, n-butanol and H₂O soluble fractions. Among those fractions the ethyl acetate soluble fraction showed the most potent DPPH radical scavenging activity, and piperine was isolated from the ethyl acetate fraction. To know the antioxidant activity of piperine, we tested the activities of superoxide dismutase (SOD) and catalase together with oxidative stress tolerance and intracellular ROS level in Caenorhabditis elegans. To investigate whether piperine-mediated increased stress tolerance was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain including CF1553. Consequently, piperine enhanced SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose–dependent manner. Moreover, piperine-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.
