Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods, and their Cytotoxicity
10.20307/nps.2019.25.2.122
- Author:
Duc Dat LE
1
;
Duc Hung NGUYEN
;
Bing Tian ZHAO
;
Byung Sun MIN
;
Si Whan SONG
;
Mi Hee WOO
Author Information
1. Drug Research and Development Center, College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Korea. woomh@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Phlomis umbrosa;
antioxidant;
developed HPLC/PDA method;
cytotoxicity
- MeSH:
Hemorrhage;
Inhibitory Concentration 50;
Iridoids;
Methods;
Phlomis
- From:Natural Product Sciences
2019;25(2):122-129
- CountryRepublic of Korea
- Language:English
-
Abstract:
The roots of Phlomis umbrosa (Turcz.) (Phlomidis Radix) have been traditionally used to treat cold, reduce swelling and staunch bleeding. Four iridoids (1 – 3 and 5) and six phenylethanoid derivatives (4, and 6 – 10) were isolated from the roots of P. umbrosa. A simple, sensitive, and reliable analytical HPLC/PDA method was developed, validated, and applied to determine 10 marker compounds in Phlomidis Radix. Furthermore, the isolates were evaluated for cytotoxic and anti-oxidant activities as well as DPPH-HPLC method. Among them, compounds 4 and 6 – 9 displayed potent anti-oxidant capacities using DPPH assay with IC50 values of 27.7 ± 2.4, 10.2 ± 1.1, 18.0 ± 0.8, 19.1 ± 0.3, and 19.9 ± 0.6 µM, and compounds 6, 8, and 9 displayed significant cytotoxic activity against HL-60 with IC50 values of 35.4 ± 3.1, 18.6 ± 2.0, and 42.9 ± 3.0 µM, respectively.
