Evaluation of Acute and Sub-acute Oral Toxicity Effect of Aquilaria malaccensis Leaves Aqueous Extract in Male ICR Mice
10.20307/nps.2019.25.2.157
- Author:
Nur Hidayat Che MUSA
1
;
Haniza Hanim Mohd ZAIN
;
Husni IBRAHIM
;
Nor Nasibah Mohd JAMIL
Author Information
1. Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Malaysia. haniza@fsmt.upsi.edu.my
- Publication Type:Original Article
- Keywords:
Aquilaria malaccensis;
Aqueous extract;
Acute toxicity;
Sub-acute toxicity
- MeSH:
Animals;
Birth Rate;
Body Weight;
Complementary Therapies;
Humans;
Hypnotics and Sedatives;
Male;
Mice;
Mice, Inbred ICR;
Mortality;
Organ Size;
Spermatozoa;
Thymelaeaceae
- From:Natural Product Sciences
2019;25(2):157-164
- CountryRepublic of Korea
- Language:English
-
Abstract:
The study was conducted to investigate the acute and sub-acute toxicity effect of Aquilaria malaccensis leaves aqueous extract (AEAM) towards male ICR mice in terms of body weight, relative organ weight, mortality rate and sperm parameters. In acute toxicity study, a single dose at of 2000 mg/kg was performed. In sub-acute toxicity study, the mice were received normal saline (control group), 50, 100, 150, 200, 500, or 1000 mg/kg of AEAM orally for 21 days of treatment. In sub-acute toxicity study, the number of abnormal sperm were significantly decreased in AEAM 100, 150, 200, 500, and 1000 when compared to the control group. While, the motility of sperm were found to be significantly increased in AEAM 100, 150, 200, and 1000 as compared to the control group. No mortality was recorded in the control group and treated groups in both toxicity studies except for one mouse from AEAM 1000 group. However, the mild sedative effect in terms of the tendency to sleep was clearly noticeable in both toxicity studies. Results indicated that the AEAM can be one of the useful alternative medicine to enhance fertility rate by increasing healthy sperm production.
