Efficacy and local irritation evaluation of Eriobotrya japonica leaf ethanol extract
10.1186/s42826-019-0003-3
- Author:
Nak Won SEONG
1
;
Won Jun OH
;
Il Soo KIM
;
Su Jin KIM
;
Ji Eun SEO
;
Chang Eon PARK
;
Da Young KIM
;
Je Won KO
;
Jong Choon KIM
Author Information
1. Health Care Institute, Korea Testing and Research Institute, Hwasun, Jeonnam 58141, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Eriobotrya japonica leaf ethanol extract;
Anti-oxidant activity;
Anti-inflammatory activity;
Anti-melanogenic activity;
Local irritation;
Animal alternative test
- MeSH:
Animals;
Chromatography, Liquid;
Eriobotrya;
Ethanol;
In Vitro Techniques;
Melanocytes;
Nitric Oxide;
Polyphenols;
RAW 264.7 Cells;
Skin;
Superoxides;
Tannins;
Tumor Necrosis Factor-alpha
- From:Laboratory Animal Research
2019;35(1):13-22
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Although Eriobotrya japonica leaves have been studied as a raw material for various cosmetic products, little is known about the anti-oxidant, anti-inflammatory, and anti-melanogenic activities of Eriobotrya japonica leaf ethanol extract (EJEE). METHODS: This study was conducted to evaluate the anti-oxidant, anti-inflammatory, and anti-melanogenic activities of EJEE using different in vitro models. In addition, we investigated the potential irritation of EJEE to skin and eye using animal alternative tests. RESULTS: The total content of polyphenols, one of the active constituents of EJEE, was analyzed by high-performance liquid chromatography and found to contain 88.68 mg tannic acid equivalent/g. EJEE showed a concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging activity, and a superoxide dismutase-like activity. The anti-inflammatory effect of 0.5% (w/v) EJEE was demonstrated by a reduction in lipopolysaccharide-induced nitric oxide and tumor necrosis factor-alpha levels in RAW 264.7 cells. EJEE also significantly inhibited melanogenesis in melanocyte stimulating hormone-induced B16F1 cells. EJEE did not show any irritation in skin and eye in animal alternative test. CONCLUSIONS: These results indicate that the EJEE possesses anti-oxidant, anti-inflammatory, and anti-melanogenic activities, while it did not induce toxicity or irritation in neither skin nor eye. Therefore, EJEE can be used as a cosmetic ingredient for skin improvement.
