Beta-carotene prevents the spermatogenic disorders induced by exogenous scrotal hyperthermia through modulations of oxidative stress, apoptosis, and androgen biosynthesis in mice
10.14405/kjvr.2019.59.2.59
- Author:
Jung Min YON
1
;
Jae Seung KIM
;
Chunmei LIN
;
Seul Gi PARK
;
Lee Wha GWON
;
Jong Geol LEE
;
In Jeoung BAEK
;
Sang Seop NAHM
;
Sang Yoon NAM
Author Information
1. College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea. synam@cbu.ac.kr
- Publication Type:Original Article
- Keywords:
β-carotene;
ellagic acid;
spermatogenic disorders;
scrotal hyperthermia;
testes
- MeSH:
Adult;
Animals;
Apoptosis;
Baths;
beta Carotene;
Caspase 3;
Ellagic Acid;
Fever;
Fruit;
Germ Cells;
Glutathione Peroxidase;
Hot Temperature;
Humans;
Hydrogen Peroxide;
Infertility, Male;
Leydig Cells;
Male;
Mice;
Oxidative Stress;
Oxidoreductases;
Superoxide Dismutase;
Testis;
Testosterone;
Vegetables;
Water;
Weight Loss
- From:Korean Journal of Veterinary Research
2019;59(2):59-67
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated whether β-carotene (β-CA) or ellagic acid (EA), originating from various fruits and vegetables, has a preventive effect against male infertility induced by exogenous scrotal hyperthermia. ICR adult mice were intraperitoneally treated with 10 mg/kg of β-CA or EA daily for 13 days consecutively. During this time, mice were subjected to transient scrotal heat stress in a water bath at 43℃ for 20 min on day 7, and their testes and blood were obtained on day 14 for histopathologic and biochemical analyses. Heat stress induced significant testicular weight reduction, germ cell loss and degeneration, as well as abnormal localization of phospholipid hydroperoxide glutathione peroxidase (PHGPx) and manganese superoxide dismutase (MnSOD) in spermatogenic and Leydig cells. Heat stress also altered the levels of oxidative stress (lipid peroxidation, SOD activity, and PHGPx, MnSOD, and HIF-1α mRNAs), apoptosis (Bax, Bcl-xL, caspase 3, NF-κB, and TGF-β1 mRNAs), and androgen biosynthesis (serological testosterone concentration and 3β-hydroxysteroid dehydrogenase mRNA) in testes. These changes were all improved significantly by β-CA treatment, but only slightly improved by EA treatment. These findings indicate that β-CA, through modulations of oxidative stress, apoptosis, and androgen biosynthesis, is a potent preventive agent against testicular injuries induced by scrotal hyperthermia.
