Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles
10.14405/kjvr.2019.59.2.81
- Author:
Hanna LEE
1
;
Yongjin LEE
;
Joohyeong LEE
;
Geun Shik LEE
;
Seung Tae LEE
;
Eunsong LEE
Author Information
1. College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea. eslee@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
in vitro maturation;
macromolecules;
oocyte growth;
small antral follicles
- MeSH:
Blastocyst;
Embryonic Development;
Female;
Follicular Fluid;
Glutathione;
In Vitro Techniques;
Metaphase;
Oocytes;
Parthenogenesis;
Polyvinyl Alcohol;
Pregnancy;
Serum Albumin, Bovine
- From:Korean Journal of Veterinary Research
2019;59(2):81-88
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small antral follicles (SAF). Immature oocytes were cultured for two days in IVG medium supplemented with 10% (v/v) fetal bovine serum (FBS), 10% (v/v) pig follicular fluid (PFF), 0.4% (w/v) bovine serum albumin (BSA), or 0.1% (w/v) polyvinyl alcohol (PVA) and then maintained for 44 h for maturation. After IVG, the mean diameters of the SAF treated with FBS, PVA, and no IVG-MAF (113.0–114.8 µm) were significantly larger than that of no IVG-SAF (111.8 µm). The proportion of metaphase II oocytes was higher in PFF (73.6%) than in BSA (43.5%) and PVA (53.7%) but similar to that in the FBS treatment (61.5%). FBS and PFF increased cumulus expansion significantly compared to PVA and BSA while the intraoocyte glutathione content was not influenced by the macromolecules. Blastocyst formation of PA oocytes treated with FBS (51.8%), PFF (50.4%), and PVA (45.2%) was significantly higher than that of the BSA-treated oocytes (20.6%). These results show that the PFF and FBS treatments during IVG improved the growth, maturation, and embryonic development of SAF.
