Anti-melanogenesis and Anti-inflammatory Effects of Cinnamomi Cortex Extract In Vitro
- Author:
Ju Hee PARK
1
;
Su Jung PARK
;
Kui Young PARK
;
Beom Joon KIM
Author Information
1. Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea. kyky@medimail.co.kr, beomjoon@unitel.co.kr
- Publication Type:Original Article
- Keywords:
Anti-inflammatory agents;
Cinnamomi cortex;
Melanin
- MeSH:
Anti-Inflammatory Agents;
Asia;
Blotting, Western;
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Europe;
Herbal Medicine;
Hyperpigmentation;
In Vitro Techniques;
Inflammation;
Melanins;
Melanoma;
Melanoma, Experimental;
Microphthalmia-Associated Transcription Factor;
Monophenol Monooxygenase;
Nitric Oxide;
RAW 264.7 Cells;
Skin Diseases
- From:Korean Journal of Dermatology
2019;57(8):448-456
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Many researchers have sought to identify safe, natural herbal extracts that exert an anti-melanogenesis effect. Cinnamomi cortex has been widely used as a herbal medicine in Asia and Europe. OBJECTIVE: To confirm the inhibitory effects of Cinnamomi cortex extract against melanogenesis and inflammation and to elucidate the underlying mechanism of these actions. METHODS: Effects of Cinnamomi cortex extract on melanin synthesis and tyrosinase activity in B16F10 melanoma cells were evaluated using an ELISA reader. Tyrosinase and MITF protein expression was determined using western blotting. Nitric oxide production in RAW 264.7 cells was measured using Griess reaction. PGE₂ was assayed with an ELISA kit. RESULTS: Cinnamomi cortex extracts inhibited melanin synthesis, tyrosinase activity, and MITF and tyrosinase expression through regulation of the ERK and CREB genes in α-MSH-induced B16 melanoma cells. In addition, Cinnamomi cortex extracts inhibited the expression of NO, PGE₂, and pro-inflammatory cytokines in lipopolysaccharide-induced RAW 264.7 cells. CONCLUSION: We suggest that Cinnamomi cortex may be a potentially useful agent for treating inflammatory skin diseases such as hyperpigmentation based on its inhibitory effects against melanin synthesis and inflammation response in vitro.
