Enhanced protective immune response to PCV2 adenovirus vaccine by fusion expression of Cap protein with InvC in pigs
- Author:
Zhencang ZHANG
1
;
Yan LUO
;
Yanming ZHANG
;
Kangkang GUO
Author Information
- Publication Type:Original Article
- Keywords: Swine; circovirus; adenovirus vaccines; immunization; capsid protein
- MeSH: Adenoviridae; Adenovirus Vaccines; Antibodies; Antibodies, Neutralizing; Capsid; Capsid Proteins; Circovirus; Genetic Engineering; Immunization; Interleukin-13; Lung; Lymph Nodes; Lymphocytes; Swine; Yersinia pseudotuberculosis
- From:Journal of Veterinary Science 2019;20(4):e35-
- CountryRepublic of Korea
- Language:English
- Abstract: The major immunogenic protein capsid (Cap) of porcine circovirus type 2 (PCV2) is critical to induce neutralizing antibodies and protective immune response against PCV2 infection. This study was conducted to investigate the immune response of recombinant adenovirus expressing PCV2b Cap and C-terminal domain of Yersinia pseudotuberculosis invasin (Cap-InvC) fusion protein in pigs. The recombinant adenovirus rAd-Cap-InvC, rAd-Cap and rAd were generated and used to immunize pigs. The phosphate-buffered saline was used as negative control. The specific antibodies levels in rAd-Cap-InvC and ZJ/C-strain vaccine groups were higher than that of rAd-Cap group (p < 0.05), and the neutralization antibody titer in rAd-Cap-InvC group was significantly higher than those of other groups during 21–42 days post-immunization (DPI). Moreover, lymphocyte proliferative level, interferon-γ and interleukin-13 levels in rAd-Cap-InvC group were increased compared to rAd-Cap group (p < 0.05). After virulent challenge, viruses were not detected from the blood samples in rAd-Cap-InvC and ZJ/C-strain vaccine groups after 49 DPI. And the respiratory symptom, rectal temperature, lung lesion and lymph node lesion were minimal and similar in the ZJ/C-strain and rAd-Cap-InVC groups. In conclusion, our results demonstrated that rAd-Cap-InvC was more efficiently to stimulate the production of antibody and protect pigs from PCV2 infection. We inferred that InvC is a good candidate gene for further development and application of PCV2 genetic engineering vaccine.
