Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm
- Author:
Dimas A ABDILLAH
1
;
Erif M. N. SETYAWAN
;
Hyun Ju OH
;
Kihae RA
;
Seok Hee LEE
;
Min Jung KIM
;
Byeong Chun LEE
Author Information
- Publication Type:Original Article
- Keywords: Dogs; Sperm; Cryopreservation; Reactive oxygen species; Protamine
- MeSH: Acrosome; Animals; B-Lymphocytes; Cryopreservation; Dogs; Male; Reactive Oxygen Species; Sperm Motility; Spermatozoa
- From:Journal of Veterinary Science 2019;20(1):79-86
- CountryRepublic of Korea
- Language:English
- Abstract: The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly increased sperm motility from that in the control (p < 0.05). Lower mitochondrial reactive oxygen species (ROS) modulator (ROMO1) and pro-apoptotic gene (BAX) expressions, together with higher expressions of protamine-2 (PRM2), protamine-3 (PRM3), anti-apoptotic B-cell lymphoma-2 (BCL2), and sperm acrosome associated-3 (SPACA3) genes were detected in the iodixanol-treated group. In addition, decreased protamine deficiency and cryocapacitation were observed in the treatment group. Our results show that supplementation with 1.5% iodixanol is ideal for reducing production of ROS and preventing detrimental effects during the canine sperm cryopreservation process, effects manifested as increased motility and reduced cryocapacitation in frozen-thawed spermatozoa.
