Genetic identification of astroviruses in wild boars
- Author:
Stefan VILCEK
1
;
Slavomira SALAMUNOVA
;
Anna JACKOVA
Author Information
- Publication Type:Brief Communication
- Keywords: Wild boar; Porcine astrovirus; Chicken astrovirus; Bat astrovirus; Phylogenetic analysis
- MeSH: Avastrovirus; Chickens; Reverse Transcriptase Polymerase Chain Reaction; Sus scrofa; Swine
- From:Journal of Veterinary Science 2019;20(1):91-94
- CountryRepublic of Korea
- Language:English
- Abstract: Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15.0%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analyzed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), 2 as lineage 4 (PAstV-4), 2 identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to a bat astrovirus lineage. This first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.
