Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy
10.4142/jvs.2018.19.5.620
- Author:
Sevil ATALAY VURAL
1
;
Rifki HAZIROGLU
;
Mehmet R VURAL
;
Ibrahim M POLAT
;
Arda S TUNC
Author Information
1. Department of Pathology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey. sevilvural@yahoo.com
- Publication Type:Original Article
- Keywords:
antineoplastic agent;
immunohistochemistry;
long interspersed nuclear element-1;
venereal tumors
- MeSH:
Animals;
B-Lymphocytes;
Cytoplasm;
Dogs;
Drug Therapy;
Eosinophils;
Immunity, Humoral;
Immunohistochemistry;
Polymerase Chain Reaction;
Retroelements;
Transfection;
Transforming Growth Factor beta;
Venereal Tumors, Veterinary
- From:Journal of Veterinary Science
2018;19(5):620-626
- CountryRepublic of Korea
- Language:English
-
Abstract:
Canine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long interspersed nuclear elements (LINE-1) retrotransposon has a pivotal role in allogenic transfection among uncontrolled dog populations. This study aimed to perform pathomorphological, immunohistochemical, and in situ polymerase chain reaction (PCR) evaluation of CTVT (n = 18) in transfected dogs during chemotherapy. Immunohistochemically, tumor phases were investigated by using specific markers (CD3, CD4, CD8, CD79, and transforming growth factor beta [TGF-β]), and investigated an amplified specific sequence of TVT LINE-1 retrotransposon by in situ PCR. Polyhedral-shaped neoplastic cells that had large, round, hypo/hyperchromatic nuclei and eosinophilic cytoplasm were detected. All marker results were positive, especially in the early weeks of recovery. CD4 and TGF-β markers were conspicuously positive at the initial stage. In situ PCR LINE-1 sequence was initially positive in only four cases. It is believed that the CD and TGF-β markers provide phase identification at tumor initiation and during chemotherapy. It is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, is needed so that regression of the tumor is possible.
