Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection
10.4142/jvs.2018.19.5.643
- Author:
Mwense LEYA
1
;
Won Kyong KIM
;
Jeong Sang CHO
;
Eun Chae YU
;
Young Jee KIM
;
Yoonhwan YEO
;
Kwang Soo LYOO
;
Myeon Sik YANG
;
Sang Seop HAN
;
John Hwa LEE
;
Dongseob TARK
;
Jin HUR
;
Bumseok KIM
Author Information
1. College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. bskims@jbnu.ac.kr
- Publication Type:Original Article
- Keywords:
Brucella vaccine;
Salmonella vector;
brucellosis;
goats;
protective efficacy
- MeSH:
Animals;
Bacterial Load;
Brucella abortus;
Brucella Vaccine;
Brucella;
Brucellosis;
Goats;
Immunoglobulin G;
Kidney;
Livestock;
Lung;
Proline;
Real-Time Polymerase Chain Reaction;
Salmonella typhimurium;
Salmonella;
Spleen;
Stem Cells;
Superoxides;
Vaccination
- From:Journal of Veterinary Science
2018;19(5):643-652
- CountryRepublic of Korea
- Language:English
-
Abstract:
Salmonella is an intracellular pathogen with a cellular infection mechanism similar to that of Brucella, making it a suitable choice for use in an anti-Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for four heterologous Brucella antigens (Brucella lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 10⁹ colony-forming unit (CFU)/mL (Group B) and 5 × 10¹⁰ CFU/mL (Group C) and challenged the inoculations with virulent Brucella abortus at 6 weeks post-immunization. Serum immunoglobulin G titers against individual antigens in Salmonella immunized goats (Group C) were significantly higher than those of the non-immunized goats (Group A) at 3 and 6 weeks after vaccination. Upon antigenic stimulation, interferon-γ from peripheral blood mononuclear cells was significantly elevated in Groups B and C compared to that in Group A. The immunized goats had a significantly higher level of protection as demonstrated by the low bacterial loads in most tissues from the goats challenged with B. abortus. Relative real-time polymerase chain reaction results revealed that the expression of Brucella antigens was lower in spleen, kidney, and lung of immunized goats than of non-immunized animals. Also, treatment with our combination vaccine ameliorated histopathological lesions induced by the Brucella infection. Overall, the Salmonella Typhimurium delivery-based combination vaccine was effective in delivering immunogenic Brucella proteins, making it potentially useful in protecting livestock from brucellosis.
