Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer
10.4142/jvs.2018.19.4.492
- Author:
Anukul TAWEECHAIPAISANKUL
1
;
Geon A KIM
;
Jun Xue JIN
;
Su Cheong YEOM
;
Byeong Chun LEE
Author Information
1. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. clee@snu.ac.kr
- Publication Type:Original Article
- Keywords:
heterograft;
miniature swine;
somatic cell nuclear transfer
- MeSH:
Adult;
Cell Line;
Cytidine;
DNA;
Ear;
Embryonic Development;
Female;
Fibroblasts;
Flow Cytometry;
Fluorescent Antibody Technique;
Genetics;
Heterografts;
Humans;
Kidney;
Korea;
Physiology;
Pregnancy;
Skin;
Swine;
Swine, Miniature;
Transplantation, Heterologous
- From:Journal of Veterinary Science
2018;19(4):492-499
- CountryRepublic of Korea
- Language:English
-
Abstract:
Due to their similarities with humans in anatomy, physiology, and genetics miniature pigs are becoming an attractive model for biomedical research. We aim to establish and evaluate blood type O cells derived from Korean native pig (KNP), a typical miniature pig breed in Korea. Ten cell lines derived from 8 KNP piglets and one adult female KNP (kidney and ear tissues) were established. To confirm the presence of blood type O, genomic DNA, fucosyltransferase (FUT) expression, and immunofluorescence staining were examined. Additionally, fluorescence-activated cell sorting and somatic cell nuclear transfer were performed to investigate the normality of the cell lines and to evaluate their effectiveness in embryo development. We found no significant bands corresponding to specific blood group A, and no increase in FUT expression in cell lines derived from piglets No. 1, No. 4, No. 5, No. 8, and the adult female KNP; moreover, they showed normal levels of expression of α 1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase. There was no significant difference in embryo development between skin and kidney fibroblasts derived from the blood type O KNPs. In conclusion, we successfully established blood type O KNP cell lines, which may serve as a useful model in xenotransplantation research.
