An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
10.4142/jvs.2018.19.4.519
- Author:
Ji Eun YU
1
;
In Ohk OUH
;
Hyeonjeong KANG
;
Hye young LEE
;
Kwang Myun CHEONG
;
In Soo CHO
;
Sang Ho CHA
Author Information
1. Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea. virusmania@korea.kr
- Publication Type:Original Article
- Keywords:
immunochromatographic assay;
on-farm detection;
porcine reproductive and respiratory syndrome virus
- MeSH:
Agriculture;
Antibodies;
Colloids;
Communicable Diseases;
Diagnosis;
Enzyme-Linked Immunosorbent Assay;
Fluorescent Antibody Technique;
Genetic Variation;
Gold Colloid;
Immunoassay;
Immunochromatography;
Immunoglobulin M;
Methods;
Nucleocapsid Proteins;
Open Reading Frames;
Porcine Reproductive and Respiratory Syndrome;
Porcine respiratory and reproductive syndrome virus;
Sensitivity and Specificity;
Swine
- From:Journal of Veterinary Science
2018;19(4):519-527
- CountryRepublic of Korea
- Language:English
-
Abstract:
Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus (PRRSV)-specific antibodies. The method utilized colloidal gold nanoparticle-labeled dual-type nucleocapsid proteins encoded by open reading frame 7. We evaluated 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs. Based on true PRRSV-specific antibody-positive or
