A Salmonella Typhi ghost induced by the E gene of phage φX174 stimulates dendritic cells and efficiently activates the adaptive immune response
10.4142/jvs.2018.19.4.536
- Author:
Gayeon WON
1
;
Seong Kug EO
;
Sang Youel PARK
;
Jin HUR
;
John Hwa LEE
Author Information
1. College of Veterinary Medicine, Chonbuk National University, Iksan Campus, Iksan 54596, Korea. johnhlee@jbnu.ac.kr
- Publication Type:Original Article
- Keywords:
Salmonella Typhi;
dendritic cells;
innate immunity
- MeSH:
Adaptive Immunity;
Animals;
Bacteriophages;
Dendritic Cells;
Immunity, Innate;
In Vitro Techniques;
Interferons;
Interleukin-4;
Interleukins;
Major Histocompatibility Complex;
Mice;
Salmonella typhi;
Salmonella;
T-Lymphocytes;
Th2 Cells;
Typhoid Fever
- From:Journal of Veterinary Science
2018;19(4):536-542
- CountryRepublic of Korea
- Language:English
-
Abstract:
Previously, we genetically engineered a Salmonella Typhi bacterial ghost (STG) as a novel inactivated vaccine candidate against typhoid fever. The underlying mechanism employed by the ghost in stimulating the adaptive immune response remains to be investigated. In this study, we aimed to evaluate the immunostimulatory effect of STG on mouse bone marrow-derived dendritic cells (BMDCs) and its activation of the adaptive immune response in vitro. Immature BMDCs were stimulated with STG, which efficiently stimulated maturation events in BMDCs, as indicated by upregulated expressions of CD40, CD80, and major histocompatibility complex class II molecules on CD11⁺ BMDCs. Immature BMDCs responded to STG stimulation by significantly increasing the expression of interleukin (IL)-6, which might indicate the induction of dendritic cell maturation in vivo (p < 0.05). In addition, ghost-stimulated murine BMDCs showed significant expressions of interferon gamma and IL-4, which can drive the development of Th1 and Th2 cells, respectively, in co-cultured CD4⁺ T cells in vitro. These results suggest that STG can effectively stimulate maturation of BMDCs and facilitate subsequent immune responses via potent immunomodulatory cytokine responses.
