Association between endotoxin levels in dust from indoor swine housing environments and the immune responses of pigs
10.4142/jvs.2018.19.3.331
- Author:
Katharine ROQUE
1
;
Kyung Min SHIN
;
Ji Hoon JO
;
Gyeong Dong LIM
;
Eun Seob SONG
;
So Jung SHIN
;
Ravi GAUTAM
;
Jae Hee LEE
;
Yeon Gyeong KIM
;
Ah Rang CHO
;
Chang Yul KIM
;
Hyun Ji KIM
;
Myung Sook LEE
;
Hyeong Geu OH
;
Byung Chul LEE
;
Jung Hee KIM
;
Kwang Ho KIM
;
Hyun Kyu JEONG
;
Hyoung Ah KIM
;
Yong HEO
Author Information
1. Department of Occupational Health, College of Bio-Medical Sciences, Daegu Catholic University, Gyeongsan 38430, Korea. yheo@cu.ac.kr
- Publication Type:Original Article
- Keywords:
cellular immunity;
endotoxins;
organic dust;
swine
- MeSH:
Agriculture;
Animal Husbandry;
Animals;
Blood Cell Count;
Concanavalin A;
Cross-Sectional Studies;
Cytokines;
Dust;
Endotoxins;
Horseshoe Crabs;
Housing;
Immunity, Cellular;
Immunoglobulin A;
Immunoglobulin E;
Immunoglobulin G;
Immunoglobulins;
In Vitro Techniques;
Interferon-gamma;
Interleukin-4;
Interleukins;
Jugular Veins;
Korea;
Lymphocytes;
Methods;
Neutrophils;
Plasma;
Swine;
Tumor Necrosis Factor-alpha
- From:Journal of Veterinary Science
2018;19(3):331-338
- CountryRepublic of Korea
- Language:English
-
Abstract:
Indoor animal husbandry environments are inevitably contaminated with endotoxins. Endotoxin exposure is associated with various inflammatory illnesses in animals. This cross-sectional study evaluated the relationship between the degree of endotoxin exposure and the cellular and humoral immune profiles of fattening pigs. Blood samples were taken from the jugular vein of 47 pigs from ten pig farms in Korea. Whole blood cell counts and plasma immunoglobulin (Ig) classes were determined. Peripheral-blood mononuclear cells were stimulated in vitro with concanavalin A for 48 h, and cytokines released into culture supernatants were measured. The barns in which the pigs lived were assessed for endotoxin levels in the total and respirable dust by using the limulus amebocyte lysate kinetic QCL method. Low and high endotoxin exposures were defined as ≤ 30 and > 30 EU/m³, respectively. Compared to pigs with low endotoxin exposure (n = 19), highly exposed pigs (n = 28) had higher circulating neutrophil and lymphocyte (particularly B cells) counts, IgG and IgE levels, interferon-gamma (IFNγ) and interleukin (IL)-4 productions, and lower IgA levels and tumor necrosis factor-alpha (TNFα) production. The IL-4, IFNγ, and TNFα levels significantly correlated with endotoxin level and/or pig age. Constant exposure of pigs to high levels of airborne endotoxins can lead to aberrant immune profiles.
