Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China
10.4142/jvs.2018.19.3.339
- Author:
Xiukun SUI
1
;
Ting XIN
;
Xiaoyu GUO
;
Hong JIA
;
Ming LI
;
Xintao GAO
;
Jing WU
;
Yitong JIANG
;
L WILLEMS
;
Hongfei ZHU
;
Shaohua HOU
Author Information
1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. houshaohua@126.com
- Publication Type:Original Article
- Keywords:
genomics;
phylogeny;
porcine respiratory and reproductive syndrome virus;
virulence
- MeSH:
Asian Continental Ancestry Group;
China;
Genomics;
Humans;
Mortality;
Phylogeny;
Porcine Reproductive and Respiratory Syndrome;
Porcine respiratory and reproductive syndrome virus;
Vaccines;
Virulence
- From:Journal of Veterinary Science
2018;19(3):339-349
- CountryRepublic of Korea
- Language:English
-
Abstract:
Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation site (N³³→K³³), and a K151 amino acid (AA) substitution for virulence in the GP5 of FZ16A were detected; except the 30 AA deletion in the Nsp2-coding region. Inoculation experiments were conducted and weaker virulence of FZ16A than FZ06A was observed. Based on our results, a 30 AA deletion in the Nsp2-coding region is an unreliable genomic indicator of a high virulence PRRSV strain. The Nsp2 and GP5 differences, in addition to the virulence difference between these two highly pathogenic PRRSV strains, have the potential to be used to establish a basis for further study of PRRSV virulence determinants and to provide data useful in the development of vaccines against this economically devastating disease.
