Identification, sequence analysis, and infectivity of H9N2 avian influenza viruses isolated from geese
10.4142/jvs.2018.19.3.406
- Author:
Rui ZHU
1
;
Xueqin YANG
;
Jianjun ZHANG
;
Danwen XU
;
Jiawen FAN
;
Huoying SHI
;
Shifeng WANG
;
Xiufan LIU
Author Information
1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. hyshi@yzu.edu.cn
- Publication Type:Original Article
- Keywords:
H9N2;
biological characteristics;
chickens;
geese;
infection
- MeSH:
Animals;
Asian Continental Ancestry Group;
Chickens;
Geese;
Humans;
Influenza A Virus, H7N9 Subtype;
Influenza A Virus, H9N2 Subtype;
Influenza in Birds;
Neuraminidase;
Population Characteristics;
Poultry;
Sequence Analysis;
Seroconversion;
Vaccination
- From:Journal of Veterinary Science
2018;19(3):406-415
- CountryRepublic of Korea
- Language:English
-
Abstract:
The subtype H9N2 avian influenza virus greatly threatens the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three H9N2 virus strains, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the Gs/JS/YZ527/11 virus is a double-reassortant virus, combining genes of A/Quail/Hong Kong/G1/97 (H9N2, G1/97)-like and A/Chicken/Shanghai/F/98 (H9N2, F/98)-like; the Gs/JS/SQ119/12 virus is a triple-reassortant virus combining genes of G1/97-like, F/98-like, and A/Duck/Shantou/163/2004 (H9N2, ST/163/04)-like. The sequences of Gs/JS/JD564/12 share high homology with those of the F/98 virus, except for the neuraminidase gene, whereas the internal genes of Gs/JS/YZ527/11 and Gs/JS/SQ119/12 are closely related to those of the H7N9 viruses. An infectivity analysis of the three isolates showed that Gs/JS/SQ119/12 and Gs/JS/YZ527/11 replicated well, with seroconversion, in geese and chickens, the Gs/JS/JD564/12 did not infect well in geese or chickens, and the F/98 virus only infected chickens, with seroconversion. Emergence of these new reassortant H9N2 avian influenza viruses indicates that these viruses can infect both chicken and goose and can produce different types of lesions in each species.
