Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
- Author:
Sharad SAURABH
1
;
Shubhankar SIRCAR
;
Jobin J KATTOOR
;
Souvik GHOSH
;
Nobumichi KOBAYASHI
;
Krisztian BANYAI
;
Obli R VINODHKUMAR
;
Ujjwal K DE
;
Nihar R SAHOO
;
Kuldeep DHAMA
;
Yashpal S MALIK
Author Information
- Publication Type:Original Article
- Keywords: enterotoxins; nsp4 gene evolution; porcine; rotavirus; viral nonstructural proteins
- MeSH: Animals; Classification; Clone Cells; Enterotoxins; Epitopes; Genetic Variation; Genotype; Humans; Protein Folding; Protein Processing, Post-Translational; Rotavirus; Sequence Analysis; Viral Nonstructural Proteins; Virulence
- From:Journal of Veterinary Science 2018;19(1):35-43
- CountryRepublic of Korea
- Language:English
- Abstract: Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes were amplified, cloned, sequenced, and then analyzed for antigenic epitopes, RotaC classification, homology, genetic relationship, modeling of NSP4 protein, and prediction of post-translational modification. RV presence was observed in both diarrheic and non-diarrheic piglets. All nsp4 genes possessed the E1 genotype. Comparison of primary, secondary, and tertiary structure and the prediction of post-translational modifications of NSP4 from diarrheic and non-diarrheic piglets revealed no apparent differences. Sequence analysis indicated that nsp4 genes have a multi-phyletic evolutionary origin and exhibit species independent genetic diversity. The results emphasize the evolution of the E9 nsp4 genotype from the E1 genotype and suggest that the diarrhea-inducing capability of porcine RVs may not be exclusively linked to its enterotoxin gene.
