Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis
- Author:
Satoshi GONDAIRA
1
;
Hidetoshi HIGUCHI
;
Hidetomo IWANO
;
Koji NISHI
;
Takanori NEBU
;
Keiichi NAKAJIMA
;
Hajime NAGAHATA
Author Information
- Publication Type:Original Article
- Keywords: Mycoplasma bovis; bovine mammary epithelial cells; cytokines; innate immunity; microarray analysis
- MeSH: Agriculture; Bacteria; Cytokines; Efficiency; Epithelial Cells; Escherichia coli; Female; Gene Expression Profiling; Histones; Immunity, Innate; Interleukin-6; Interleukins; Mastitis; Microarray Analysis; Mycoplasma bovis; Mycoplasma; RNA, Messenger; Staphylococcus aureus; Toll-Like Receptors; Tumor Necrosis Factor-alpha
- From:Journal of Veterinary Science 2018;19(1):79-87
- CountryRepublic of Korea
- Language:English
- Abstract: Mycoplasma spp. are contagious bacteria, and mycoplasmal mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMECs) have an important role in the elimination of pathogens, but the effect of Mycoplasma bovis on bMECs has not been fully described. To elucidate the immune response against intramammary infection by M. bovis, we undertook microarray analysis to examine and profile mRNA expression in bMECs after stimulation with M. bovis. We also compared the effects of M. bovis, Staphylococcus aureus, and Escherichia coli on immune-related mRNA expression in bMECs. Transcriptome analysis indicated a significant decrease in the level of mRNA-encoding lysine-specific demethylase 4D, suggesting that the immune response is suppressed by a decrease in histone demethylase activity. Interleukin (IL)-1β, IL-6, tumor necrosis factor alpha, toll-like receptor (TLR) 2, and TLR4 mRNA expression levels were significantly increased in bMECs stimulated with heat-killed M. bovis, but the expression levels were lower than those following stimulation by heat-killed S. aureus or E. coli. Our results suggest that M. bovis weakly affects mRNA expression in bMECs compared to the effects of E. coli or S. aureus. Moreover, live M. bovis may induce suppression of the immune response in bMECs.
