The effects of silicone on mononuclear cell blastogenesis and il-1beta/tnf-alphasecretion of monocyte in human
- Author:
NO AUTHORS LISTED
- Publication Type:In Vitro
- MeSH:
Allergy and Immunology;
Blood Donors;
Chemotaxis;
Enzyme-Linked Immunosorbent Assay;
Healthy Volunteers;
Humans;
Inflammation;
Lymphocyte Activation;
Monocytes;
Rheumatic Diseases;
Silicones;
Tumor Necrosis Factor-alpha
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
1998;25(7):1226-1235
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Although there are numerous reviews of clinical and epidemiologic data, there has been no critical analysis of silicone immunology. The purpose of this study is to identify human celluar immune reaction to silicone through mononuclear cell blastogenesis as well as by measuring IL-1betaand TNF-alpha released from human monocyte/macrophage incubated with silicone in vitro. In the study, total 14 healthy volunteers participated in the experiment as blood donors. in the peripheral blood mononuclear cell blastogenesis assay, one control group and three experimental groups were designed. The three experimental groups were composed of a silicone treated group, a silicone/phytohemagglutinin treated group, and a phytohemagglutinin treated group. The peripheral blood mononuclear cells were isolated with the Ficoll-Hypaque gradient method, and they were incubated for 72 hours. The proliferation of the peripheral blood mononuclear cells was measured with the [3H]-thymidme uptake. In the cytokine assay of monocyte stimulated by silicone, human monocyte was isolated from the peripheral blood mononuclear cells through the magnetic cell sorting(MACS) method. One control group and three experimental groups were designed also in the experiment. The experimental groups were composed of a silicone treated group, a silicone/lipopolysaccharide treated group, and a lipopolysaccharide treated group. The monocytes of each group were incubated for 1,3,6,24 and 48 hours. The supernatants were preserved at
