The Elevated Expression of the High Mobility Group-I(Y) Proteins in Thyroid Cancer using Semi-Quantitation RT-PCR.
- Author:
Jin Woo RYU
1
;
Sang Jin KIM
Author Information
1. Department of Surgery, College of Medicine, Dankook University.
- Publication Type:Original Article
- Keywords:
Thyroid cancer;
HMGI(Y);
Semi-quantified RT-PCR
- MeSH:
Adenoma;
Alternative Splicing;
Genes, rRNA;
Goiter;
HMGA1a Protein;
Humans;
Nuclear Proteins;
Thyroid Gland*;
Thyroid Neoplasms*
- From:Journal of the Korean Surgical Society
1998;54(1):23-27
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
High Mobility Group I(HMG-I) proteins are nuclear proteins that are required for induction of the human IFN-beta gene by virus and for the regulation of the tumor necrosis-beta factor and rRNA genes. Proteins I and Y result from alternative splicing of a single functional gene named HMGI(Y). In several studies, elevated expressions of the HMGI proteins (HMGI, HMGY, and HMGI-C) have been used as markers in thyroid cancer, but not in adenomas, goiters, and normal thyroid tissues and cells. Here, we try to demonstrate the elevated expression of the HMGI(Y) proteins in thyroid carcinomas by using semi-quantified RT-PCR (Reverse Transcription and Polymerase Chain Reaction). In cases of thyroid carcinomas 4 of 5(80%) were positive, in 10 cases of adenomas, goiters, and normal thyroid tissues, 1(10%) was positive. These results suggest that the semi-quantified RT-PCR is useful preoperative diagnostic tool for differentiating thyroid tumors.
