A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media.
10.7704/kjhugr.2017.17.4.208
- Author:
Jung Hwan LEE
1
;
Jiwan PARK
;
Mi Ri PARK
;
Yoon Hee NA
;
Soo Jeong CHO
Author Information
1. Center for Gastric Cancer, National Cancer Center, Goyang, Korea. crystal522@daum.net
- Publication Type:Comparative Study ; Original Article
- Keywords:
Agar;
Culture;
Helicobacter pylori;
Media
- MeSH:
Agar;
Brain;
Brucella;
Cacao;
Heart;
Helicobacter pylori*;
Helicobacter*;
Horses
- From:The Korean Journal of Helicobacter and Upper Gastrointestinal Research
2017;17(4):208-212
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Optimal culture conditions for Helicobacter pylori have not been established. We compared the effectiveness of four different agar-based media for the growth of H. pylori. MATERIALS AND METHODS: G27, ATCC #43504 and 60190, and primary cultured strains were used. H. pylori strains were cultured for four days under four culture conditions: chocolate agar, Thayer-Martin (TM) agar containing vancomycin-colistin-nystatin inhibitor (VCNI), Brucella agar, and brain heart infusion (BHI) agar containing 5% horse blood and IsoVitaleX (BBL™ BD, USA). Culture of cells in each medium was repeated fourteen times. The growth of H. pylori was measured by using a spectrophotometer. RESULTS: TM, Brucella, and BHI agars showed mean absorbance values of 0.099, 0.059, 1.410, and 0.913, respectively. These values were significantly different (P=0.030). After post-adjustment by Bonferroni correction, similar growth was noted for in chocolate, Brucella, and BHI agars; however, TM agar significantly suppressed H. pylori growth compared with Brucella agar (P=0.031). CONCLUSIONS: Chocolate, Brucella, and BHI agars provided effective culture conditions for the growth of H. pylori. TM agar containing VCNI suppressed the growth of H. pylori and other organisms.
