Study PML/RAR alpha fusion gene on 21 patients diagnosed with acute promyelocytic leukemia (AML \u2013 M3)
- Author:
Phuong Minh Vu
;
Vinh Quang Pham
;
Hoa Khanh Bach
;
Nhung Thi Hong Le
;
Dung Thi My Tran
;
Phuong Minh Nguyen
- Publication Type:Journal Article
- Keywords:
Acute promyelocytic leukemia;
M3;
PML/RAR alpha
- MeSH:
Acute promyelocytic leukemia;
M3;
PML/RAR alpha
- From:Journal of Medical Research
2008;58(5):18-24
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: In Vietnam, there are a number of studies on the application of ATRA in treating acute promyelocytic leukemia (AML \u2013 M3) but they have still faced with certain difficulties. Objectives: (1). Study PML/RAR alpha fusion gene on the patients diagnosed with AML \u2013 M3. (2). Study the index of hematology of the PML/RAR alpha positive group. Subject and Method: 21 patients with acute promyelocytic leukemia (M3) were studied. All patients were examined with morphology, coagulation and cytogenetic tests and RNA were extracted from leukemic cells and PCR for PML/RAR alpha fusion transcript. Result and conclusion: PML/RAR alpha positive in 67% including 4 patients which were not discovered t(15; 17) by cytogenetic technique. Rates of three subtype (bcr1, bcr2 and bcr3) of PML/RAR alpha were 7 patients (50%), 3 patients (21,5%) and 4 patients (28,5%), respectively. WBC and bone marrow cells of PML/RAR alpha positive group were 5.08+/-3.87 and 155.82+/-106.21. D \u2013 Dimer level was 1954.89+/-1575.28; 93% of patients in the PML/RAR alpha positive group had DIC.
