Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants.

Lei Yang; Xiaohui Zhang; Liren Wang; Shuming Yin; Biyun Zhu; Ling Xie; Qiuhui Duan; Huiqiong HU; Rui Zheng; Yu Wei; Liangyue Peng; Honghui Han; Jiqin Zhang; Wenjuan Qiu; Hongquan Geng; Stefan Siwko; Xueli Zhang; Mingyao Liu; Dali LI

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Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants.

Author: Lei YANG ¹ ; Xiaohui ZHANG ¹ ; Liren WANG ¹ ; Shuming YIN ¹ ; Biyun ZHU ¹ ; Ling XIE ¹ ; Qiuhui DUAN ¹ ; Huiqiong HU ¹ ; Rui ZHENG ² ; Yu WEI ¹ ; Liangyue PENG ¹ ; Honghui HAN ³ ; Jiqin ZHANG ¹ ; Wenjuan QIU ² ; Hongquan GENG ² ; Stefan SIWKO ⁴ ; Xueli ZHANG ¹ ; Mingyao LIU ¹ ; Dali LI ⁵
Author Information

1. East China Normal University and Shanghai Fengxian District Central Hospital Joint Center for Translational Medicine, Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
2. Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
3. Bioray Laboratories Inc., Shanghai, 200241, China.
4. Department of Molecular and Cellular Medicine, The Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, TX, 77030, USA.
5. East China Normal University and Shanghai Fengxian District Central Hospital Joint Center for Translational Medicine, Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, 200241, China. dlli@bio.ecnu.edu.cn.
Publication Type:Letter
MeSH: Adenosine; genetics; Adenosine Deaminase; genetics; metabolism; Animals; CRISPR-Associated Protein 9; genetics; metabolism; CRISPR-Cas Systems; genetics; Embryo, Mammalian; metabolism; Gene Editing; Genetic Variation; genetics; Mice; Mice, Inbred C57BL; Rats; Rats, Sprague-Dawley
From: Protein & Cell 2018;9(9):814-819
CountryChina
Language:English