Dgcr8 deletion in the primitive heart uncovered novel microRNA regulating the balance of cardiac-vascular gene program.
10.1007/s13238-018-0572-1
- Author:
Xi CHEN
1
;
Lin WANG
1
;
Rujin HUANG
1
;
Hui QIU
1
;
Peizhe WANG
1
;
Daren WU
2
;
Yonglin ZHU
1
;
Jia MING
1
;
Yangming WANG
2
;
Jianbin WANG
3
;
Jie NA
4
Author Information
1. Center for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing, 100084, China.
2. Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing, 100871, China.
3. School of Life Sciences, Tsinghua University, Beijing, 100084, China.
4. Center for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing, 100084, China. jie.na@tsinghua.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cardiovascular progenitor cells;
Dgcr8;
Single cell RNA sequencing;
miRNA-541;
microRNA
- From:
Protein & Cell
2019;10(5):327-346
- CountryChina
- Language:English
-
Abstract:
Primitive mammalian heart transforms from a single tube to a four-chambered muscular organ during a short developmental window. We found that knocking out global microRNA by deleting Dgcr8 microprocessor in Mesp1 cardiovascular progenitor cells lead to the formation of extremely dilated and enlarged heart due to defective cardiomyocyte (CM) differentiation. Transcriptome analysis revealed unusual upregulation of vascular gene expression in Dgcr8 cKO hearts. Single cell RNA sequencing study further confirmed the increase of angiogenesis genes in single Dgcr8 cKO CM. We also performed global microRNA profiling of E9.5 heart for the first time, and identified that miR-541 was transiently highly expressed in E9.5 hearts. Interestingly, introducing miR-541 back into microRNA-free CMs partially rescued their defects, downregulated angiogenesis genes and significantly upregulated cardiac genes. Moreover, miR-541 can target Ctgf and inhibit endothelial function. Our results suggest that microRNAs are required to suppress abnormal angiogenesis gene program to maintain CM differentiation.
