PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.

Xiaona Huang; Chao Wei; Fenjie LI; Lumeng Jia; Pengguihang Zeng; Jiahe LI; Jin Tan; Tuanfeng Sun; Shaoshuai Jiang; Jia Wang; Xiuxiao Tang; Qingquan Zhao; Bin Liu; Limin Rong; Cheng LI; Junjun Ding

Return

PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.

Author: Xiaona HUANG ¹ ; Chao WEI ¹ ; Fenjie LI ¹ ; Lumeng JIA ² ; Pengguihang ZENG ¹ ; Jiahe LI ¹ ; Jin TAN ¹ ; Tuanfeng SUN ¹ ; Shaoshuai JIANG ¹ ; Jia WANG ¹ ; Xiuxiao TANG ¹ ; Qingquan ZHAO ¹ ; Bin LIU ³ ; Limin RONG ³ ; Cheng LI ² ; Junjun DING ⁴
Author Information

1. RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.
2. Center for Bioinformatics, School of Life Sciences, Peking University, Beijing, 100871, China.
3. Department of Spine Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
4. RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China. dingjunj@mail.sysu.edu.cn.
Publication Type:Journal Article
Keywords: 3D chromatin; PCGF6; Polycomb group; pluripotency; super-enhancer
From: Protein & Cell 2019;10(10):709-725
CountryChina
Language:English
Abstract: Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression. However, the mechanism underlying the activation function of PCGF6 is poorly understood. Here, we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as OCT4. In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression. By combining with promoter capture Hi-C data, we found that PCGF6 activates cell cycle genes by regulating SE-promoter interactions via 3D chromatin. Our findings highlight a novel mechanism of PcG protein in regulating pluripotency, and provide a research basis for the therapeutic application of pluripotent stem cells.