Phage display screening of therapeutic peptide for cancer targeting and therapy.
10.1007/s13238-019-0639-7
- Author:
Phei Er SAW
1
;
Er-Wei SONG
2
Author Information
1. Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China.
2. Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China. songew@mail.sysu.edu.cn.
- Publication Type:Journal Article
- Keywords:
over-expressed receptor;
phage display;
tumor microenvironment;
tumor stromal cells;
tumor targeting peptide;
tumor vasculature
- From:
Protein & Cell
2019;10(11):787-807
- CountryChina
- Language:English
-
Abstract:
Recently, phage display technology has been announced as the recipient of Nobel Prize in Chemistry 2018. Phage display technique allows high affinity target-binding peptides to be selected from a complex mixture pool of billions of displayed peptides on phage in a combinatorial library and could be further enriched through the biopanning process; proving to be a powerful technique in the screening of peptide with high affinity and selectivity. In this review, we will first discuss the modifications in phage display techniques used to isolate various cancer-specific ligands by in situ, in vitro, in vivo, and ex vivo screening methods. We will then discuss prominent examples of solid tumor targeting-peptides; namely peptide targeting tumor vasculature, tumor microenvironment (TME) and over-expressed receptors on cancer cells identified through phage display screening. We will also discuss the current challenges and future outlook for targeting peptide-based therapeutics in the clinics.
