Dynamic roles of angiopoietin-like proteins 1, 2, 3, 4, 6 and 7 in the survival and enhancement of ex vivo expansion of bone-marrow hematopoietic stem cells.
10.1007/s13238-013-2066-5
- Author:
Shahina AKHTER
1
;
Md Mashiar RAHMAN
;
Hyun Seo LEE
;
Hyeon-Jin KIM
;
Seong-Tshool HONG
Author Information
1. Department of Microbiology and Genetics and Institute for Medical Science, Chonbuk National University Medical School, Jeonju, 561-712, South Korea.
- Publication Type:Journal Article
- MeSH:
Angiopoietin-like 4 Protein;
Angiopoietin-like Proteins;
Angiopoietins;
genetics;
metabolism;
Animals;
Antigens, Ly;
metabolism;
Bone Marrow Cells;
cytology;
CHO Cells;
Cell Differentiation;
drug effects;
Cell Lineage;
Cell Proliferation;
drug effects;
Cell Survival;
drug effects;
Cells, Cultured;
Cricetinae;
Cricetulus;
Culture Media, Conditioned;
pharmacology;
Hematopoietic Stem Cells;
cytology;
metabolism;
Intercellular Signaling Peptides and Proteins;
pharmacology;
Membrane Proteins;
metabolism;
Mice;
Transfection
- From:
Protein & Cell
2013;4(3):220-230
- CountryChina
- Language:English
-
Abstract:
Recent advances in hematopoietic stem cells (HSCs) expansion by growth factors including angiopoietin-like proteins (Angptls) have opened up the possibility to use HSCs in regenerative medicine. However, the unavailability of true in vitro HSCs expansion by these growth factors has limited the understanding of the cellular and molecular mechanism of HSCs expansion. Here, we report the functional role of mouse Angptls 1, 2, 3, 4, 6 and 7 and growth factors SCF, TPO, IGF-2 and FGF-1 on purified mouse bone-marrow (BM) Lineage(-)Sca-1(+)(Lin-Sca-1(+)) HSCs. The recombinant retroviral transduced-CHO-S cells that secrete Angptls in serum-free medium were used alone or in combination with growth factors (SCF, TPO, IGF-2 and FGF-1). None of the Angptls stimulated HSC proliferation, enhanced or inhibited HSCs colony formation, but they did support the survival of HSCs. By contrast, any of the six Angptls together with saturating levels of growth factors dramatically stimulated a 3- to 4.5-fold net expansion of HSCs compared to stimulation with a combination of those growth factors alone. These findings lead to an understanding of the basic function of Angptls on signaling pathways for the survival as well as expansion of HSCs in the bone marrow niche.
