A transcription assay for EWS oncoproteins in Xenopus oocytes.
10.1007/s13238-010-0114-y
- Author:
King Pan NG
1
;
Felix CHEUNG
;
Kevin A W LEE
Author Information
1. Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon Hong Kong, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biological Assay;
Female;
Oncogene Proteins;
genetics;
Oncogenes;
genetics;
Oocytes;
metabolism;
pathology;
RNA-Binding Protein EWS;
genetics;
metabolism;
Sarcoma, Ewing;
genetics;
pathology;
Xenopus
- From:
Protein & Cell
2010;1(10):927-934
- CountryChina
- Language:English
-
Abstract:
Aberrant chromosomal fusion of the Ewing's sarcoma oncogene (EWS) to several different cellular partners produces the Ewing's family of oncoproteins (EWS-fusion-proteins, EFPs) and associated tumors (EFTs). EFPs are potent transcriptional activators, dependent on the N-terminal region of EWS (the EWS-activation-domain, EAD) and this function is thought to be central to EFT oncogenesis and maintenance. Thus EFPs are promising therapeutic targets, but detailed molecular studies will be pivotal for exploring this potential. Such studies have so far largely been restricted to intact mammalian cells while recent evidence has indicated that a mammalian cell-free transcription system may not support bona fide EAD function. Therefore, the lack of manipulatable assays for the EAD presents a significant barrier to progress. Using Xenopus laevis oocytes we describe a plasmid-based micro-injection assay that supports efficient, bona fide EAD transcriptional activity and hence provides a new vehicle for molecular dissection of the EAD.
