Generation of glyco-engineered BY2 cell lines with decreased expression of plant-specific glycoepitopes.
10.1007/s13238-011-1007-4
- Author:
Bo-Jiao YIN
1
;
Ting GAO
;
Nuo-Yan ZHENG
;
Yin LI
;
San-Yuan TANG
;
Li-Ming LIANG
;
Qi XIE
Author Information
1. State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing, 100101, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Blotting, Western;
Carbohydrate Sequence;
Cell Line;
Cloning, Molecular;
DNA, Complementary;
genetics;
Down-Regulation;
Epitopes;
genetics;
immunology;
Fucose;
metabolism;
Fucosyltransferases;
chemistry;
deficiency;
genetics;
immunology;
Glycoproteins;
chemistry;
genetics;
immunology;
Molecular Sequence Data;
Pentosyltransferases;
chemistry;
deficiency;
genetics;
immunology;
Polysaccharides;
chemistry;
immunology;
Protein Engineering;
methods;
RNA Interference;
Species Specificity;
Tobacco;
cytology;
genetics;
Xylose;
metabolism
- From:
Protein & Cell
2011;2(1):41-47
- CountryChina
- Language:English
-
Abstract:
Plants are known to be efficient hosts for the production of mammalian therapeutic proteins. However, plants produce complex N-glycans bearing β1,2-xylose and core α1,3-fucose residues, which are absent in mammals. The immunogenicity and allergenicity of plant-specific Nglycans is a key concern in mammalian therapy. In this study, we amplified the sequences of 2 plant-specific glycosyltransferases from Nicotiana tabacum L. cv Bright Yellow 2 (BY2), which is a well-established cell line widely used for the expression of therapeutic proteins. The expression of the endogenous xylosyltranferase (XylT) and fucosyltransferase (FucT) was downregulated by using RNA interference (RNAi) strategy. The xylosylated and core fucosylated N-glycans were significantly, but not completely, reduced in the glycoengineered lines. However, these RNAi-treated cell lines were stable and viable and did not exhibit any obvious phenotype. Therefore, this study may provide an effective and promising strategy to produce recombinant glycoproteins in BY2 cells with humanized N-glycoforms to avoid potential immunogenicity.
