Applications of RNA interference high-throughput screening technology in cancer biology and virology.
10.1007/s13238-014-0076-6
- Author:
Shan GAO
1
;
Chen YANG
;
Shan JIANG
;
Xiao-Ning XU
;
Xin LU
;
You-Wen HE
;
Annie CHEUNG
;
Hui WANG
Author Information
1. Department of Oncology, John Radcliffe Hospital, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, UK, shan.gao@oncology.ox.ac.uk.
- Publication Type:Journal Article
- MeSH:
Animals;
Genetic Predisposition to Disease;
genetics;
Humans;
Molecular Targeted Therapy;
methods;
trends;
Neoplasms;
genetics;
therapy;
RNA Interference;
Viral Proteins;
genetics;
Virus Diseases;
genetics;
therapy;
virology
- From:
Protein & Cell
2014;5(11):805-815
- CountryChina
- Language:English
-
Abstract:
RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.
