USP33, a new player in lung cancer, mediates Slit-Robo signaling.
10.1007/s13238-014-0070-z
- Author:
Pushuai WEN
1
;
Ruirui KONG
;
Jianghong LIU
;
Li ZHU
;
Xiaoping CHEN
;
Xiaofei LI
;
Yongzhan NIE
;
Kaichun WU
;
Jane Y WU
Author Information
1. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Cell Line, Tumor;
Cell Movement;
genetics;
physiology;
Cohort Studies;
Down-Regulation;
Female;
Gene Expression Regulation, Neoplastic;
HEK293 Cells;
Humans;
Immunohistochemistry;
Intercellular Signaling Peptides and Proteins;
metabolism;
Kaplan-Meier Estimate;
Lung Neoplasms;
genetics;
metabolism;
pathology;
Male;
Middle Aged;
Nerve Tissue Proteins;
metabolism;
Prognosis;
RNA Interference;
Receptors, Immunologic;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
genetics;
physiology;
Ubiquitin Thiolesterase;
genetics;
metabolism
- From:
Protein & Cell
2014;5(9):704-713
- CountryChina
- Language:English
-
Abstract:
Ubiquitin specific protease 33 (USP33) is a multifunctional protein regulating diverse cellular processes. The expression and role of USP33 in lung cancer remain unexplored. In this study, we show that USP33 is down-regulated in multiple cohorts of lung cancer patients and that low expression of USP33 is associated with poor prognosis. USP33 mediates Slit-Robo signaling in lung cancer cell migration. Downregulation of USP33 reduces the protein stability of Robo1 in lung cancer cells, providing a previously unknown mechanism for USP33 function in mediating Slit activity in lung cancer cells. Taken together, USP33 is a new player in lung cancer that regulates Slit-Robo signaling. Our data suggest that USP33 may be a candidate tumor suppressor for lung cancer with potential as a prognostic marker.
