The recombinant expression systems for structure determination of eukaryotic membrane proteins.
10.1007/s13238-014-0086-4
- Author:
Yuan HE
1
;
Kan WANG
;
Nieng YAN
Author Information
1. State Key Laboratory of Bio-membrane and Membrane Biotechnology, Tsinghua university, Beijing, 100084, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Escherichia coli;
genetics;
Eukaryotic Cells;
metabolism;
Genetic Vectors;
HEK293 Cells;
Humans;
Insecta;
cytology;
genetics;
Membrane Proteins;
chemistry;
genetics;
metabolism;
Recombinant Proteins;
chemistry;
metabolism;
Yeasts;
genetics
- From:
Protein & Cell
2014;5(9):658-672
- CountryChina
- Language:English
-
Abstract:
Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryotic membrane protein structures have been obtained due to the technical challenges in the generation of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.
