Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains.
10.1007/s13238-014-0060-1
- Author:
Peipei LIU
1
;
Di LIU
;
Xi YANG
;
Jing GAO
;
Yan CHEN
;
Xue XIAO
;
Fei LIU
;
Jing ZOU
;
Jun WU
;
Juncai MA
;
Fangqing ZHAO
;
Xuyu ZHOU
;
George F GAO
;
Baoli ZHU
Author Information
1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China, peipei.liu@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Complementarity Determining Regions;
genetics;
DNA Primers;
chemistry;
genetics;
Female;
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor;
genetics;
Gene Rearrangement, delta-Chain T-Cell Antigen Receptor;
genetics;
Genes, T-Cell Receptor beta;
genetics;
Genetic Variation;
High-Throughput Nucleotide Sequencing;
Humans;
Immunoglobulin Joining Region;
genetics;
Immunoglobulin Variable Region;
genetics;
Male;
Receptors, Antigen, T-Cell, alpha-beta;
genetics
- From:
Protein & Cell
2014;5(8):603-615
- CountryChina
- Language:English
-
Abstract:
The characterization of the human T-cell receptor (TCR) repertoire has made remarkable progress, with most of the work focusing on the TCRβ chains. Here, we analyzed the diversity and complexity of both the TCRα and TCRβ repertoires of three healthy donors. We found that the diversity of the TCRα repertoire is higher than that of the TCRβ repertoire, whereas the usages of the V and J genes tended to be preferential with similar TRAV and TRAJ patterns in all three donors. The V-J pairings, like the V and J gene usages, were slightly preferential. We also found that the TRDV1 gene rearranges with the majority of TRAJ genes, suggesting that TRDV1 is a shared TRAV/DV gene (TRAV42/DV1). Moreover, we uncovered the presence of tandem TRBD (TRB D gene) usage in ~2% of the productive human TCRβ CDR3 sequences.
