A study of miRNAs targets prediction and experimental validation.
10.1007/s13238-010-0129-4
- Author:
Yong HUANG
1
;
Quan ZOU
;
Haitai SONG
;
Fei SONG
;
Ligang WANG
;
Guozheng ZHANG
;
Xingjia SHEN
Author Information
1. Jiang Su University of Science and Technology, Zhenjiang 212018, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Computational Biology;
methods;
Gene Expression Regulation;
Humans;
MicroRNAs;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Validation Studies as Topic
- From:
Protein & Cell
2010;1(11):979-986
- CountryChina
- Language:English
-
Abstract:
microRNAs (miRNAs) are 20-24 nucleotide (nt) RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs (mRNAs). To identify miRNA target genes will help a lot by understanding their biological functions. Sophisticated computational approaches for miRNA target prediction, and effective biological techniques for validating these targets now play a central role in elucidating their functions. Owing to the imperfect complementarity of animal miRNAs with their targets, it is difficult to judge the accuracy of the prediction. Complexity of regulation by miRNA-mediated targets at protein and mRNAs levels has made it more challenging to identify the targets. To date, only a few miRNAs targets are confirmed. In this article, we review the methods of miRNA target prediction and the experimental validation for their corresponding mRNA targets in animals.
