MiR-29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6.
10.1007/s13238-016-0277-2
- Author:
Kegan ZHU
1
;
Lei LIU
2
;
Junliang ZHANG
1
;
Yanbo WANG
3
;
Hongwei LIANG
3
;
Gentao FAN
1
;
Zhenhuan JIANG
2
;
Chen-Yu ZHANG
4
;
Xi CHEN
5
;
Guangxin ZHOU
6
Author Information
1. Department of Orthopedics, School of Medicine, Jinling Hospital, Nanjing University, Nanjing, 210002, China.
2. Department of Orthopedics, The Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, China.
3. State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, NJU Advanced Institute for Life Sciences (NAILS), Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China.
4. State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, NJU Advanced Institute for Life Sciences (NAILS), Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China. cyzhang@nju.edu.cn.
5. State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, NJU Advanced Institute for Life Sciences (NAILS), Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China. xichen@nju.edu.cn.
6. Department of Orthopedics, School of Medicine, Jinling Hospital, Nanjing University, Nanjing, 210002, China. oxis@163.com.
- Publication Type:Journal Article
- Keywords:
miR-29b;
migration;
osteosarcoma;
proliferation;
tumorigenesis
- MeSH:
3' Untranslated Regions;
Animals;
Base Sequence;
Bone Neoplasms;
metabolism;
pathology;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Cyclin-Dependent Kinase 6;
antagonists & inhibitors;
genetics;
metabolism;
Humans;
Mice;
MicroRNAs;
metabolism;
Osteosarcoma;
metabolism;
pathology;
RNA Interference;
RNA, Messenger;
metabolism;
RNA, Small Interfering;
metabolism;
Rats;
Sequence Alignment;
Up-Regulation
- From:
Protein & Cell
2016;7(6):434-444
- CountryChina
- Language:English
-
Abstract:
Osteosarcoma is the most common primary sarcoma of bone, and it is a leading cause of cancer death among adolescents and young adults. However, the molecular mechanism underlying osteosarcoma carcinogenesis remains poorly understood. Recently, cyclin-dependent kinase 6 (CDK6) was identified as an important oncogene. We found that CDK6 protein level, rather than CDK6 mRNA level, is much higher in osteosarcoma tissues than in normal adjacent tissues, which indicates a post-transcriptional mechanism involved in CDK6 regulation in osteosarcoma. MiRNAs are small non-coding RNAs that repress gene expression at the post-transcriptional level and have widely been shown to play important roles in many human cancers. In this study, we investigated the role of miR-29b as a novel regulator of CDK6 using bioinformatics methods. We demonstrated that CDK6 can be downregulated by miR-29b via binding to the 3'-UTR region in osteosarcoma cells. Furthermore, we identified an inverse correlation between miR-29b and CDK6 protein levels in osteosarcoma tissues. Finally, we examined the function of miR-29b-driven repression of CDK6 expression in osteosarcoma cells. The results revealed that miR-29b acts as a tumor suppressor of osteosarcoma by targeting CDK6 in the proliferation and migration processes. Taken together, our results highlight an important role for miR-29b in the regulation of CDK6 in osteosarcoma and may open new avenues for future osteosarcoma therapies.
