Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells.
- Author:
Jiaming SU
1
;
Yi SUI
1
;
Jian DING
1
;
Fuqiang LI
1
;
Shuang SHEN
1
;
Yang YANG
1
;
Zeming LU
1
;
Fei WANG
1
;
Lingling CAO
1
;
Xiaoxia LIU
2
;
Jingji JIN
3
;
Yong CAI
4
Author Information
1. School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.
2. Department of Gynecologic Oncology, The First Clinical Hospital of Jilin University, 71 Xinmin Street, Changchun, 130021, China.
3. School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, 130012, China. jjjin@jlu.edu.cn.
4. School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, 130012, China. caiyong62@jlu.edu.cn.
- Publication Type:Journal Article
- Keywords:
BCCIP;
chromatin remodeling complex;
human INO80
- MeSH:
Calcium-Binding Proteins;
genetics;
metabolism;
Cell Cycle Proteins;
genetics;
metabolism;
Chromatin Assembly and Disassembly;
physiology;
DNA Helicases;
genetics;
metabolism;
HeLa Cells;
Humans;
Multiprotein Complexes;
genetics;
metabolism;
Nuclear Proteins;
genetics;
metabolism;
Promoter Regions, Genetic;
physiology;
Transcription, Genetic;
physiology;
YY1 Transcription Factor;
genetics;
metabolism
- From:
Protein & Cell
2016;7(10):749-760
- CountryChina
- Language:English
-
Abstract:
The BCCIP (BRCA2- and CDKN1A-interacting protein) is an important cofactor for BRCA2 in tumor suppression. Although the low expression of BCCIP is observed in multiple clinically diagnosed primary tumor tissues such as ovarian cancer, renal cell carcinoma and colorectal carcinoma, the mechanism of how BCCIP is regulated in cells is still unclear. The human INO80/YY1 chromatin remodeling complex composed of 15 subunits catalyzes ATP-dependent sliding of nucleosomes along DNA. Here, we first report that BCCIP is a novel target gene of the INO80/YY1 complex by presenting a series of experimental evidence. Gene expression studies combined with siRNA knockdown data locked candidate genes including BCCIP of the INO80/YY1 complex. Silencing or over-expressing the subunits of the INO80/YY1 complex regulates the expression level of BCCIP both in mRNA and proteins in cells. Also, the functions of INO80/YY1 complex in regulating the transactivation of BCCIP were confirmed by luciferase reporter assays. Chromatin immunoprecipitation (ChIP) experiments clarify the enrichment of INO80 and YY1 at +0.17 kb downstream of the BCCIP transcriptional start site. However, this enrichment is significantly inhibited by either knocking down INO80 or YY1, suggesting the existence of both INO80 and YY1 is required for recruiting the INO80/YY1 complex to BCCIP promoter region. Our findings strongly indicate that BCCIP is a potential target gene of the INO80/YY1 complex.
