A new method for quantifying mitochondrial axonal transport.
10.1007/s13238-016-0268-3
- Author:
Mengmeng CHEN
1
;
Yang LI
2
;
Mengxue YANG
1
;
Xiaoping CHEN
3
;
Yemeng CHEN
4
;
Fan YANG
4
;
Sheng LU
4
;
Shengyu YAO
3
;
Timothy ZHOU
3
;
Jianghong LIU
5
;
Li ZHU
5
;
Sidan DU
4
;
Jane Y WU
6
Author Information
1. University of Chinese Academy of Sciences, Beijing, 100049, China.
2. School of Electronic Science & Engineering, Nanjing University, Nanjing, 210093, China. yogo@nju.edu.cn.
3. Department of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.
4. School of Electronic Science & Engineering, Nanjing University, Nanjing, 210093, China.
5. State Key Laboratory for Brain & Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
6. State Key Laboratory for Brain & Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China. jane-wu@northwestern.edu.
- Publication Type:Journal Article
- Keywords:
FUS proteinopathy and mitochondrial transport defect;
image processing and analysis;
mitochondrial transport
- MeSH:
Animals;
Axonal Transport;
physiology;
Cerebral Cortex;
cytology;
metabolism;
Drosophila melanogaster;
cytology;
metabolism;
Embryo, Mammalian;
Gene Expression;
Lab-On-A-Chip Devices;
Microscopy, Confocal;
Mitochondria;
metabolism;
ultrastructure;
Motor Neurons;
metabolism;
ultrastructure;
Movement;
Mutation;
Primary Cell Culture;
RNA-Binding Protein FUS;
genetics;
metabolism;
Rats;
Rats, Sprague-Dawley;
Software
- From:
Protein & Cell
2016;7(11):804-819
- CountryChina
- Language:English
-
Abstract:
Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microfluidic-chamber-cultured neurons together with a newly developed analysis package named "MitoQuant". This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms (temperature changes, drug treatment and genetic manipulation) that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mitochondrial transport that were not detected by traditional kymographic analyses.
