miR-27b inhibits gastric cancer metastasis by targeting NR2F2.
10.1007/s13238-016-0340-z
- Author:
Qingzhao FENG
1
;
Xionglin WU
2
;
Fuchao LI
3
;
Beibei NING
4
;
Xiaofeng LU
5
;
Yin ZHANG
5
;
Ying PAN
6
;
Wenxian GUAN
7
Author Information
1. Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China. izaobao@vip.qq.com.
2. Department of Emergency, The Yiwu Affiliated Hospital of Wenzhou Medical University, Yiwu, 322000, China.
3. Department of Gerontology, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China.
4. The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, China.
5. Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China.
6. Department of Orthopaedics, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China.
7. Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China. guan-wx@163.com.
- Publication Type:Journal Article
- Keywords:
NR2F2;
gastric cancer;
miR-27b;
tumor metastasis
- MeSH:
Animals;
Biomarkers, Tumor;
genetics;
metabolism;
COUP Transcription Factor II;
genetics;
metabolism;
Cell Line, Tumor;
Female;
Genes, Tumor Suppressor;
Heterografts;
Humans;
Male;
Mice, Nude;
MicroRNAs;
genetics;
metabolism;
Neoplasm Metastasis;
Neoplasm Proteins;
genetics;
metabolism;
Neoplasm Transplantation;
RNA, Neoplasm;
genetics;
metabolism;
Stomach Neoplasms;
genetics;
metabolism;
pathology
- From:
Protein & Cell
2017;8(2):114-122
- CountryChina
- Language:English
-
Abstract:
Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 (NR2F2, also known as COUP-TFII) is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitro and in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells' migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells' proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients' survival.
