Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells.
10.1007/s13238-012-2938-0
- Author:
Du WEN
1
;
Yanhong XUE
;
Kuo LIANG
;
Tianyi YUAN
;
Jingze LU
;
Wei ZHAO
;
Tao XU
;
Liangyi CHEN
Author Information
1. National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium;
metabolism;
Cytoplasmic Granules;
metabolism;
Diabetes Mellitus;
metabolism;
pathology;
Disease Models, Animal;
Dynamins;
metabolism;
Electric Capacitance;
Endocytosis;
physiology;
Insulin;
metabolism;
Insulin-Secreting Cells;
metabolism;
pathology;
Male;
Mice;
Mice, Inbred C57BL;
Patch-Clamp Techniques;
Photolysis;
Primary Cell Culture
- From:
Protein & Cell
2012;3(8):618-626
- CountryChina
- Language:English
-
Abstract:
Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca(2+) entry and suppressed by the inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice, which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability.
