Mass spectrometry based proteomics, background, status and future needs.
10.1007/s13238-012-2079-5
- Author:
Peter ROEPSTORFF
1
Author Information
1. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark. roe@bmb.sdu.dk
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Electrophoresis, Gel, Two-Dimensional;
Mass Spectrometry;
Peptides;
analysis;
chemistry;
Phosphorylation;
Protein Isoforms;
analysis;
Proteome;
analysis;
Proteomics
- From:
Protein & Cell
2012;3(9):641-647
- CountryChina
- Language:English
-
Abstract:
An overview of the background for proteomics and a description of the present state of art are given with a description of the main strategies in proteomics. The advantages and limitations of the two major strategies, 2D-gel based and LC-MS based, are discussed and a combination for the two, CeLC-MS is described. A number of challenging problems which have been solved using different proteomics strategies including the advantage of organell enrichment or modifications specific peptide isolation to get deeper into the proteome are described. Finally the present status and future needs discussed.
