Find and replace: editing human genome in pluripotent stem cells.
10.1007/s13238-011-1132-0
- Author:
Huize PAN
1
;
Weiqi ZHANG
;
Weizhou ZHANG
;
Guang-Hui LIU
Author Information
1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Chromosomes, Artificial, Bacterial;
genetics;
Deoxyribonucleases;
genetics;
Dependovirus;
genetics;
Gene Targeting;
methods;
Genetic Engineering;
methods;
Genetic Vectors;
Genome, Human;
Humans;
Mutagenesis, Insertional;
Mutation;
Plasmids;
Pluripotent Stem Cells;
cytology;
metabolism;
Zinc Fingers;
genetics
- From:
Protein & Cell
2011;2(12):950-956
- CountryChina
- Language:English
-
Abstract:
Genetic manipulation of human pluripotent stem cells (hPSCs) provides a powerful tool for modeling diseases and developing future medicine. Recently a number of independent genome-editing techniques were developed, including plasmid, bacterial artificial chromosome, adeno-associated virus vector, zinc finger nuclease, transcription activator-like effecter nuclease, and helper-dependent adenoviral vector. Gene editing has been successfully employed in different aspects of stem cell research such as gene correction, mutation knock-in, and establishment of reporter cell lines (Raya et al., 2009; Howden et al., 2011; Li et al., 2011; Liu et al., 2011b; Papapetrou et al., 2011; Sebastiano et al., 2011; Soldner et al., 2011; Zou et al., 2011a). These techniques combined with the utility of hPSCs will significantly influence the area of regenerative medicine.
