Evaluation of Mycobacterial Recovery by Specimen Preparation and Inoculating Media.
- Author:
Joseph JEONG
1
;
Chulhun L CHANG
Author Information
1. Department of Clinical Pathology, College of Medicine, Pusan National University. cchl@hyowon.cc.pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium tuberculosis;
media;
specimen processing;
culture;
sputum
- MeSH:
Agar;
Alkalies;
Korea;
Mycobacterium tuberculosis;
Sputum;
Tuberculosis
- From:Korean Journal of Clinical Pathology
2000;20(2):188-193
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: During the specimen preparation, neutralization after alkali processing is not necessary when inoculate into agar or broth media, but into 3% Ogawa media, the mostly used egg-based media in Korea. To simplify the specimen processing, the recovery of mycobacteria from 3% Ogawa media inoculating differently processed sputa, was evaluated. Futhermore, the recovery from the three different media was evaluated, too. METHODS: 209 sputa were included. Each specimen was divided into 3 bottles.(A) One was only decontaminated by 4% NaOH before inoculation.(B) Another was decontaminated, and concentrated without neutralization. These two specimens were inoculated into 3% Ogawa media.(C) The other was decontaminated, neutralized and concentrated, and innoculated into 3% Ogawa, Middlebrook 7H9 selective broth and 7H10 selective agar media. The isolates were identified by AccuProbe method. RESULTS: M. tuberculosis were isolated from 43(20.5%) specimens. The recovery number and time from Ogawa media, inoculating specimens processed by A, B, and C method, were 29(13.9%), 35(16.7%), and 36(17.2%), and 23, 23, and 19 days, respectively. The recovery number and time from Ogawa, 7H10 agar and 7H9 broth media, inoculating specimens processed by C method, were 36(17.2%), 42(20.1%), and 41(19.6%), and 26, 24, and 22 days, respectively. CONCLUSIONS: Neutralization of alkali-decontaminated specimens could be used to inoculate into 3% Ogawa media. Inoculation into homemade 7H10 agar and 7H9 broth media in addition to 3% Ogawa media improved the recovery rate and time of mycobacteria.