Effects of p38 MAPK signal pathway inhibition on upper lip scar hyperplasia at different time in rabbits
10.3760/cma.j.issn.1671-0290.2019.03.020
- VernacularTitle:不同时间p38MAPK信号通路受阻对兔上唇瘢痕增生的影响
- Author:
Kaining JIA
1
;
Yaoxiang XU
;
Guo YU
;
Jin YUE
;
Feng LIU
;
Wenlin XIAO
Author Information
1. 青岛大学附属医院口腔科 青岛大学口腔医学院 266555
- Keywords:
Rabbits;
Cleft lip;
Cicatrix;
hypertrophic;
p38MAPK signaling pathway;
Gene silencing;
Adenovirus vector
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2019;25(3):243-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of p38MAPK gene silencing recombinant adeno-virus on the expression of target gene in different time and to detect the effect of p 38MAPK signal pathway on the upper lip scar hyperplasia at different time to determine the optimal scar treatment time .Methods The adenovirus vector was injected into the scar tissue in 0 week ,1 week and 2 week after cheiloplasty in rabbit .The specimens were harvested in 3 week postoperatively .Four methods in-cluding Sirius red staining ,immunohistochemical staining (IHC) ,Western blotting (WB) ,real-time PC (RT-PCR) were used to quantitatively and quantitatively detect the relative expression levels of p38MAPK and scar-related factors (col Ⅰ ,col Ⅲ ,MM P1 ,TIMP1) .Results Sirius red staining and immunohistochemical staining showed that in 1st week the expression of col Ⅲ and MMP1 in scar tis-sue was significantly higher than that in 0 week and 2 week after operation and the expression of col Ⅰand TIMP1 was significantly less than that in 0 week and 2 week after operation .The results of WB and RT-PCR were consistent with that of IHC .Conclusions After injection into the upper lip scar tis-sue with adenovirus in 1 week ,the degree of scar hyperplasia is the least .
