Current status of the reference system for measurement of serum high- and low-density lipoprotein cholesterol
10.3760/cma.j.issn.1009-8158.2019.08.004
- VernacularTitle:高密度和低密度脂蛋白胆固醇测定参考系统现状及有关问题
- Author:
Jun DONG
1
;
Wenxiang CHEN
Author Information
1. 北京医院 国家老年医学中心
- Keywords:
Cholesterol;
HDL;
Cholesterol;
LDL;
Ultracentrifugation;
Reference standards;
Reproducibility of results
- From:
Chinese Journal of Laboratory Medicine
2019;42(8):602-606
- CountryChina
- Language:Chinese
-
Abstract:
Decreased serum high-density lipoprotein cholesterol (HDL-C) and increased low-density lipoprotein cholesterol (HDL-C) concentrations are important risk factors for cardiovascular diseases. Achieving accurate and comparable HDL-C and LDL-C results is the primary requirement for the prevention and treatment of cardiovascular diseases, in which reference system including reference method, reference materials and standardization programs are needed. The current reference method for HDL-C and LDL-C measurement is the β-quantification method developed by the United States Center for Disease Control and Prevention (CDC), a multi-step procedure involving ultracentrifugation, chemical precipitation, and cholesterol analysis by CDC reference method. This method uses a large amount of serum and is technically demanding which limited its application in standardization. The reduced volume β-quantification method uses a small volume of serum sample, is simple and high through put and is more applicable. However, both methods are sensitive to serum matrix. Commutability of reference materials should be evaluated when they are used to calibrate routine methods. Fresh (non-frozen) serum samples should be used for a split sample comparison between reference and direct methods. In addition, development of reference measurement procedure based on ultracentrifugation and preparation of commutable reference materials are the important work in lipoprotein standardization.
