The development and application of nucleic acid detection techniques of infectious microorganisms in pediatrics
10.3760/cma.j.issn.1009-9158.2019.07.001
- VernacularTitle:病原体核酸技术在儿童感染性疾病检测中的发展及应用
- Author:
Wei LI
1
;
Ran TAO
;
Shiqiang SHANG
Author Information
1. 浙江大学医学院附属儿童医院实验检验中心
- Keywords:
Communicable diseases;
High-throughput nucleotide sequencing;
Child
- From:
Chinese Journal of Laboratory Medicine
2019;42(7):489-492
- CountryChina
- Language:Chinese
-
Abstract:
The advance of molecular diagnosis has evolved the field of detection of pathogens in pediatric disease towards the high-throughput ultra-sensitive molecular testing. This review provides an overview and comparison of current molecular tools on the market, including qPCR, multiplex PCR, digital PCR, isothermal amplification, and microfluidic chip, highlighting the future trends of integrated high-throughput molecular testing and automation. These evolutions will maximize the utility of limit patient samples from children and contribute significantly on the diagnosis of infectious disease.
