Application of LC-MS/MS in the accurate assessment of vitamins

VernacularTitle:液相色谱-串联质谱技术在维生素检测中的应用
Author: Xiaofen YUAN ¹ ; Jinfei MA ; Qingqing YANG ; Pengyun LIU ; Lingxiao SHEN ; Qianjun ZHANG ; Yikun LI ; Huafen LIU
Author Information

1. 杭州凯莱谱精准医疗检测技术有限公司
Keywords: Vitamins; Chromatography; liquid; Tandem mass spectrometry
From: Chinese Journal of Laboratory Medicine 2019;42(6):479-483
CountryChina
Language:Chinese
Abstract: Vitamins are classified as either fat-soluble (vitamins A, D, E, K) or water-soluble (vitamins B and vitamin C). Traditional methods of immunoassay have only been developed for vitamins D,B6, B9 and B12. However, they cannot distinguish between vitamin subtypes such as D2, D3 and associated epi isomers (which has higher leveks in infants),giving false positive or negative results. Mass spectrometry has become a gold standard method for small molecule analysis in biological samples with its advantages in speed,resolution,sensitivity and specificity. It is widely used in clinical research and diagnosis and provides an efficient method for simultaneous detection of multivitamins in one injection using one low volume sample collection.